Synthetic Low EEO Agarose (EEO 0.03, High Resolution DNA Band Separation) product detail at Eata Marisource
Synthetic Low EEO Agarose (EEO 0.03, High Resolution DNA Band Separation)
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Synthetic Low EEO Agarose (EEO 0.03, High Resolution DNA Band Separation)

Cat No:MCH-0034

Description

Agarose produced by a synthetic route independent of natural seaweed feedstock variability, delivering extremely low electroendosmosis and higher gel transparency and band resolution than conventional agarose.

Basic Information

Brand Eata Marisource
Product Grade High resolution electrophoresis grade
Chemical Name Agarose, synthetic low EEO
Raw Material Source Synthetic process, not reliant on seaweed extraction variability
Physical Form Powder
Appearance White powder
Color White
Solubility Dissolves quickly into a clear homogeneous solution without foaming
Water Solubility Clear gel of high transparency on cooling
Assay / Purity EEO 0.03; high gel strength suitable for routine and pulsed-field electrophoresis; DNase, RNase and protease free
Storage Temperature Ambient
Storage Conditions Store dry and sealed at room temperature
Intended Use High resolution nucleic acid electrophoresis including pulsed-field applications
Target Industries Molecular biology, genomics laboratories, reagent distribution
Recommended Applications Compressed sharp DNA band separation, pulsed-field gel electrophoresis, transparent imaging gels
Recommended Use Level Cast 0.8 to 2 percent gels per fragment range
Appearance of Solution Microwave to full dissolution in TAE or TBE, add stain and cast
Handling & Safety Handle as a fine powdered material; avoid dust formation and wear gloves, mask and eye protection
Chemical Class Purified agarose galactan hydrocolloid
Structural Unit Linear chain of alternating 1,3-linked beta-D-galactose and 1,4-linked 3,6-anhydro-alpha-L-galactose
Functional Groups Hydroxyl groups and bridged 3,6-anhydro rings forming helical gel junctions
Ionic Character Neutral polysaccharide with minor anionic sulfate impurities
Key Features Band separation measured nearly 30 percent better than conventional agarose in comparative gels
Functional Benefits Sharp low-diffusion bands improve quantitation and fragment calling accuracy
Typical End Products Agarose gels for DNA electrophoresis, blotting and fragment recovery
Formulation Compatibility Works in TAE and TBE systems; suited to low voltage runs around 90 V
Preparation & Dissolution Guidance Synthetic polymerisation route with defined substitution control
Reactivity & Derivatization Helix-coil transition on heating and cooling; sulfate hydrolysis only under strong acid
Stability Notes Stable dry at room temperature
Usage Precautions Hot solutions may bump; standard laboratory protection
Environmental Profile Biodegradable polysaccharide material
Analytical Methods EEO, gel strength and band resolution tested by comparative gel imaging

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