Nucleases

In the ocean, nucleic acids are recycled almost as quickly as they are made. Every strand of DNA and RNA shed by marine life is taken apart by enzymes in the water column — a quiet, constant demolition that marine organisms have been perfecting for a very long time.

Eata Marisource brings that machinery to dry land. Our nuclease range is built from two genuine marine sources: the salt-tolerant microbial strains that secrete DNA- and RNA-cleaving enzymes, and the digestive tissues of marine fish, whose endogenous nucleases are recovered rather than wasted. Each product ships with its measured activity per lot, and each can be adjusted toward the conditions your process actually runs at.

The Nuclease Range

Four working formats cover the requests we receive most often. Consider this a starting grid rather than a closed catalog — every line can be tuned.

Fig. 1 Marine microbial fermentation — where our nuclease enzyme work begins.

Enzymes That Evolved in Salt and Cold

Nucleases from terrestrial organisms are familiar tools, but marine nucleases carry a different set of traits, because the environment they evolved in demands them. Culture-based surveys of marine bacteria find that extracellular DNase production is widespread — roughly a third of cultivable isolates in one broad study secreted DNase, with the activity most common in mesophilic strains (about 54%) and prominent among genera such as Bacillus, Pseudoalteromonas and Vibrio.

Two properties stand out for practical work. First, salt tolerance: enzymes recovered from marine isolates remain active across a wide salinity range — reported working windows span roughly 80 to 1500 mM — which matters for processes that run in saline or high-ionic-strength media. Second, cold adaptation: RNases from Antarctic sea-ice bacteria such as Psychrobacter retain activity at temperatures where most terrestrial enzymes slow to a crawl, opening cleaner low-temperature processing routes.

Fig. 2 Enzyme solutions, aliquoted and ready for assay.

The Nuclease Range in Practice

Marine Microbial Nuclease. Fermented from marine bacterial strains and recovered as a defined enzyme powder. It carries both endonuclease and exonuclease activity, degrades DNA and RNA alike, and depends on magnesium — a working profile well suited to removing unwanted nucleic acids from a sample or stream.

Cold-Active Marine RNase. Derived from sea-ice microbial strains, this grade keeps its activity at low temperature and tolerates salt. For RNA work that must stay cold, it is a different class of tool from its mesophilic counterparts.

Fish-Derived Nuclease Complex. Recovered from the pyloric caeca and pancreatic tissue of marine fish such as Atlantic cod and salmon — organs that are rich in digestive enzymes and, without recovery, would simply go to waste. The complex includes DNase I-type activity with an alkaline pH optimum and operates on native and denatured DNA.

Fig. 3 Precision dispensing: the working scale of research-grade nucleases.

Measured Performance

The numbers that define a nuclease are the ones you can test. Typical parameters across the range:

Activity and purity are measured per lot; the Certificate of Analysis for your batch is the reference.

Fig. 4 Spectrophotometric checks confirm activity and purity per lot.

From Marine Source to Working Enzyme

For microbial grades, the route runs: strain isolation and characterization, fermentation under controlled conditions, harvest of the culture fluid, and purification by salting-out and chromatography — the same steps used in the classic purification of marine Vibrio nuclease, where DNase and RNase activities were shown to travel together through a single purification scheme. For fish-derived grades, the route is shorter: digestive tissues are collected from processing partners, the enzyme fraction is extracted, and the complex is dried to a stable powder.

Every step is documented, and every lot is released against its measured activity — so the enzyme you receive is defined by numbers, not by a label.

Fig. 5 A marine source in the raw: fish tissue rich in endogenous enzymes.

Where Nucleases Earn Their Keep

Fig. 6 Enzyme powder in a sealed, dry, light-protected pouch.

Documented, Lot by Lot

• Activity is the headline number. Every lot ships with its measured activity on defined substrates, so you can build your process around a number rather than a promise.
• Source transparency. Strain identity and growing conditions are recorded for microbial grades; fish species and tissue type for fish-derived grades.
• Production under recognized frameworks. ISO 9001, ISO 22000 and HACCP-based systems, with Halal and Kosher certification available on request.
• Documents that travel with the goods. COA, MSDS, certificates of origin and allergen statements are assembled with every order.

Custom Marine Enzyme Programs

Nucleases are rarely one-size-fits-all. Tell us the substrate you need to clear, the temperature and salt conditions of your process, the activity level you want per unit, and the form you can handle — we will develop samples toward it, confirm the measured activity, and scale production so the bulk enzyme matches the sample you approved.

For Research or Industrial Raw Materials, Not For Personal Medical Use!