Low Melting Point Agarose (CAS 39346-81-1, Gelling 26 to 30 C, Melting below 65 C)

Cat No:MCH-0033

Description

Modified low melting point agarose with improved molecular sieving and band clarity, melting at about 65.5 C so recovered nucleic acid bands can be remelted below nucleic acid melting points.

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Basic Information

Brand	Eata Marisource
Product Grade	Molecular biology grade
Chemical Name	Agarose, low melting point (hydroxyethylated)
Raw Material Source	Modified agarose from seaweed agar
Physical Form	Powder
Appearance	White to almost white powder
Color	White
Solubility	Dissolves on boiling in water or buffer
Water Solubility	Clear gel after cooling of hot solution
Assay / Purity	Gel strength at least 200 g per cm2 at 1 percent; EEO at most 0.10; sulfate at most 0.10 percent; DNase, RNase and protease not detected
Storage Temperature	Ambient
Storage Conditions	Store sealed at room temperature; five-year validity
Intended Use	Separation and recovery of nucleic acid fragments larger than 1000 bp, cloning directly from remelted gel slices
Target Industries	Molecular biology research, cloning and probe preparation workflows
Recommended Applications	DNA fragment recovery, radiolabelled probe preparation, plaque lifts and radial immunodiffusion protein assays
Recommended Use Level	Cast gels at 1 to 2 percent for fragments above 1000 bp
Appearance of Solution	Melt fully by boiling or microwave; remelt recovered gel slices at 65 C for downstream use
Handling & Safety	Handle as a fine powdered material; avoid dust formation and wear gloves, mask and eye protection
Chemical Class	Purified agarose galactan hydrocolloid
Structural Unit	Linear chain of alternating 1,3-linked beta-D-galactose and 1,4-linked 3,6-anhydro-alpha-L-galactose
Functional Groups	Hydroxyl groups and bridged 3,6-anhydro rings forming helical gel junctions
Ionic Character	Neutral polysaccharide with minor anionic sulfate impurities
Key Features	Melting below the denaturation temperature of most nucleic acids enables direct enzymatic use of recovered DNA; Gelling point 26 to 30 C at 1.5 percent; melting point 65 C or below at 1.5 percent
Functional Benefits	Shortens cloning workflows by allowing ligation-ready DNA straight from remelted slices
Typical End Products	Agarose gels for DNA electrophoresis, blotting and fragment recovery
Formulation Compatibility	Compatible with restriction digestion and ligation after equilibration in suitable buffer
Preparation & Dissolution Guidance	Hydroxyethylation modification of purified agarose
Reactivity & Derivatization	Helix-coil transition on heating and cooling; sulfate hydrolysis only under strong acid
Stability Notes	Five years sealed at room temperature
Usage Precautions	Molten gel can bump violently; wear heat protection and lab coat
Environmental Profile	Biodegradable polysaccharide
Analytical Methods	Gel strength, gelling and melting points, EEO and sulfate per product specification

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