Low EEO Agarose for Preparative DNA Electrophoresis (Gel Strength 1400 g per cm2, Sulfate below 0.15 Percent)

Cat No:MCH-0032

Description

Low electroendosmosis agarose of standard gel temperature and high gel strength, produced by second-generation desulfation technology with molecular-level control of gel strength and EEO.

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Basic Information

Brand	Eata Marisource
Product Grade	Genetic technology grade
Chemical Name	Agarose, low EEO
Raw Material Source	Refined agarose from seaweed agar
Physical Form	Powder
Appearance	White to light yellow powder
Color	White to light yellow
Solubility	Dissolves on boiling; forms clear gel on cooling
Water Solubility	Clear gel at 1 to 2 percent
Assay / Purity	Gel strength at least 1400 g per cm2 at 1.5 percent; EEO 0.13; sulfate content at or below 0.15 percent; viscosity at most 60 mPa s at 4 percent
Storage Temperature	Ambient
Storage Conditions	Store sealed, dry and at room temperature
Intended Use	Preparative DNA electrophoresis separating fragments from 100 bp to 15000 bp
Target Industries	Molecular biology, genetic analysis, laboratory reagent supply
Recommended Applications	Routine and preparative agarose gel electrophoresis of nucleic acids
Recommended Use Level	Cast at 0.8 to 2 percent matched to target fragment sizes
Appearance of Solution	Dissolve fully by boiling or microwaving before casting
Handling & Safety	Handle as a fine powdered material; avoid dust formation and wear gloves, mask and eye protection
Chemical Class	Purified agarose galactan hydrocolloid
Structural Unit	Linear chain of alternating 1,3-linked beta-D-galactose and 1,4-linked 3,6-anhydro-alpha-L-galactose
Functional Groups	Hydroxyl groups and bridged 3,6-anhydro rings forming helical gel junctions
Ionic Character	Neutral polysaccharide with minor anionic sulfate impurities
Key Features	DNase, RNase and protease not detected; sulphate used as the purity indicator; Gel temperature 40.0 plus or minus 2.0 C at 1.5 percent; melting temperature 90.0 plus or minus 2.0 C
Functional Benefits	Tuned gel temperature supports clean band excision for fragment recovery
Typical End Products	Agarose gels for DNA electrophoresis, blotting and fragment recovery
Formulation Compatibility	Compatible with standard TAE and TBE buffers and nucleic acid stains
Preparation & Dissolution Guidance	Second-generation desulfation of natural agarose feedstock
Reactivity & Derivatization	Helix-coil transition on heating and cooling; sulfate hydrolysis only under strong acid
Stability Notes	Stable dry at room temperature
Usage Precautions	Avoid inhaling powder; hot gel solutions can bump and spatter
Environmental Profile	Biodegradable polysaccharide
Analytical Methods	Gel strength, gel and melt temperature, viscosity, EEO and sulfate by internal grade specifications

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For Research or Industrial Raw Materials, Not For Personal Medical Use!