Agarose Low EEO Type I-B (Gel Strength 3200 g per cm2 at 1.5 Percent, EEO 0.12)

Cat No:MCH-0031

Description

Purified low electroendosmosis agarose of the linear galactan type composed of alternating beta-D-galactose and 3,6-anhydro-alpha-L-galactose units, supplied as a powder for preparative and analytical electrophoresis.

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Basic Information

Brand	Eata Marisource
Product Grade	Electrophoresis grade
Chemical Name	Agarose (3,6-anhydro-galactan)
Raw Material Source	Agar-bearing red seaweeds
Physical Form	Powder
Appearance	White powder
Color	White
Solubility	Dissolves on boiling in water or electrophoresis buffers
Water Solubility	Forms clear gel on cooling of hot solution
Assay / Purity	Gel strength 1800 g per cm2 at 1 percent and 3200 g per cm2 at 1.5 percent; EEO 0.12; ash 0.25 percent
Storage Temperature	Ambient
Storage Conditions	Store dry at room temperature
Intended Use	Nucleic acid gel electrophoresis and blotting applications
Target Industries	Molecular biology laboratories, diagnostics, reagent manufacturing
Recommended Applications	Agarose gel electrophoresis of DNA fragments, Northern and Southern blotting support media
Recommended Use Level	Cast gels at 0.8 to 2 percent according to fragment size range
Appearance of Solution	Boil or microwave to dissolve completely; cast gels at the desired concentration
Handling & Safety	Handle as a fine powdered material; avoid dust formation and wear gloves, mask and eye protection
Chemical Class	Purified agarose galactan hydrocolloid
Structural Unit	Linear chain of alternating 1,3-linked beta-D-galactose and 1,4-linked 3,6-anhydro-alpha-L-galactose
Functional Groups	Hydroxyl groups and bridged 3,6-anhydro rings forming helical gel junctions
Ionic Character	Neutral polysaccharide with minor anionic sulfate impurities
Key Features	Low EEO limits internal electroosmotic convection and sharpens nucleic acid band separation; Gel point 36 C at 1.5 percent; melting range 86.5 to 89.5 C
Functional Benefits	High gel strength allows robust gel handling with low breakage
Typical End Products	Agarose gels for DNA electrophoresis, blotting and fragment recovery
Formulation Compatibility	Compatible with TAE and TBE electrophoresis systems
Preparation & Dissolution Guidance	Agar purification, sulfate depletion and milling
Reactivity & Derivatization	Helix-coil transition on heating and cooling; sulfate hydrolysis only under strong acid
Stability Notes	Stable for years when stored dry
Usage Precautions	Molten agarose solutions are scalding hot; handle with heat protection
Environmental Profile	Biodegradable seaweed polysaccharide
Analytical Methods	Gel strength, EEO, gel point and sulfate content measured per agarose standards

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